Flow cytometric analysis of CD45 expression on human lymphocytes. Whole blood was stained with either BD Horizon™ V500 Mouse Anti-Human CD45 (Cat. No. 560777/560779; solid line histogram) or BD Horizon™ V500 Mouse IgG1, κ Isotype Control (Cat. No. 560787; dashed line histgoram). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Fluorescent histograms were derived from gated events with the side and forward light-scatter characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACSCanto™ flow cytometry system.